The only place that checkbox exists is in the All-Star Game simulation tab. I'm not sure it ever existed anywhere else.
As per the original question, I honestly don't know. There's no obvious flag of a penalty for being out of position, and there's not even clear anecdotal evidence after 55 seasons. We've always just assumed so, so no one's really tried.
For what it's worth, Bunclewirth played a lot of center and RW in his first few seasons, and he was the shit.